WebIn this study, phiC31 integrase technology in combination with DHFR amplification system was used to have an expression vector for future expression of blinatumomab in CHO cells. The gene of interest (BsAb gene) could be joined to DHFR selection marker with the insertion of an internal ribosome entry site (IRES). WebSBI’s PhiC31 Integrase System consists of the PhiC31 Integrase Expression Plasmid and a PhiC31 Donor Plasmid that has your gene-of-interest cloned in. The system enables: Non … Lentiviral Expression Plasmids & Lentiviral Vectors - PhiC31 Integrase Expression … Contact Us - PhiC31 Integrase Expression Plasmid System Biosciences Exosome Isolation Kits & Products - PhiC31 Integrase Expression Plasmid System … NTA-based Exosome Characterization - PhiC31 Integrase Expression Plasmid … Exosome Quantitation Kits & Products - PhiC31 Integrase Expression Plasmid … CLOuD9 Gene Expression Regulation System; Targeted Protein Degradation; … Lentiviral Packaging Kits - PhiC31 Integrase Expression Plasmid System Biosciences How it works. Ordering RNA Services is easy. Step 1: Contact the services team … Exosome Biomarker Discovery Products - PhiC31 Integrase Expression Plasmid … Get everything you need for studying and using exosomes, from isolation and …
PhiC31 Integrase Expression Plasmid System Biosciences
WebMay 12, 2014 · Vector constructs used in this study. (A and B) The genetic structure of the barnase expression vector (“pro-vector”) (A) and the phiC31-integrase-expression vector (B). The provector harbors attP and attB sequences that serve as targets for the site-specific recombinase phiC31. Note that only the T-DNA part of the vectors is illustrated ... Web... designed a PhiC31 integrase-based transgenesis system consisting of two components ( Fig. 1): the genomic landing site, in which EGFP is driven in the heart muscle by the zebrafish... short mountain distillery haunted woods
Site-specific chromosomal integration mediated by phiC31 integrase
WebIn addition, combining PhiC31 integrase with R4 integrase allows for isogenic expression from a defined genomic locus, providing the ideal solution for comparative analysis of gene families, isoforms or orthologs. ... Jump-In™ Fast Gateway® System allows you to generate stable clones in just two weeks by introducing your genetic material ... WebJan 1, 2007 · The phiC31 integrase system represents a novel technology that opens up new possibilities for gene therapy. The phiC31 integrase can integrate introduced plasmid … WebDec 9, 2024 · This vector system consists of several modules including a Tet-inducible promoter directing the expression of a transgene and two Tet repressor expression units placed in tandem on a single vector. The cell clones, generated using a one-step phiC31 integrase-mediated chromosomal integration of the multi-cDNA expression construct, … sans network security 2017 las vegas